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Nucleotide sequences generated in this study were submitted to GenBank. The accession numbers for these sequences are MN – MN There have been commendable efforts made towards the detection, treatment, and prevention of HIV in the country. Even though the genetic diversity of HIV has been shown to affect the parameters ranging from detection to vaccine development, there is no data available with respect to the molecular epidemiology of HIV-1 in Sri Lanka. Briefly, dried blood spot specimens DBS collected from HIV-1 infected individuals between December and August were subjected to pol gene amplification and sequencing.

These pol gene sequences were used to interpret the drug resistance mutation profiles. Our analysis revealed that the majority In addition to pure subtypes there were sequences which were observed to be circulating recombinant forms CRFs and a few of the recombinants were identified as potential unique recombinants URFs. We also observed the presence of drug resistance mutations in 56 Estimates of the Bayesian evolutionary analysis suggested that the HIV-1 subtype C was introduced to Sri Lanka during the early s The findings presented here indicate the presence of multiple HIV-1 subtypes and the prevalence of drug resistance mutations in Sri Lanka.

The majority of the sequences were subtype C, having their most recent common ancestor traced back to the early s. Continuous molecular surveillance of HIV-1 molecular epidemiology will be crucial to keep track of drug resistance, genetic diversity, and evolutionary history of HIV-1 in Sri Lanka.

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